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KMID : 0861020090240010073
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Korea Journal of Herbology
2009 Volume.24 No. 1 p.73 ~ p.78
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A Therapeutic Effect of Pinellia Ternata via the Increase of CD4+CD25+ Regulatory T Cells and the Suppression of CD3+CCR3+ Cellular Infiltration During Allergic Airway Inflammation
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Lee Young-Cheol
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Abstract
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Objectives: In this study, we studied the effect of Pinellia Ternata (PT) on regulatory T cells and CD3+CCR3+ Th2 cells number in asthma model mice.
Methods: All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2 alum-precipitated Ag containing 100 of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min, 2.5％ ovalbumin in normal saline) and PT (400, 200 mg/kg) were orally administered 3 times a week for 8 weeks. After C57BL/6 mice were orally given of PT, the percentages, cell numbers, phenotype and function of CD4+CD25+Treg cells were determined by flow cytometry.
Results: The cell numbers of CD4+CD25+Treg cell subsets were markedly increased in PT treated mice as reported. However, PT significantly reduced the CD3+CCR3+ Th2 cells in PBMC and lung of mice.
Conclusions: These results indicate that PT has a deep inhibitory effect on asthma model mice by increase the number of regulatory T cells, and by reducing CD3+CCR3+ Th2 cells.
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KEYWORD
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Pinellia Ternata (PT), asthma, regulatory T cell, CD3+CCR3+
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